Studies involving enzymic phosphorylation. 4. The conversion of d-ribose into d-ribose 5-phosphate by extracts of Escherichia coli
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منابع مشابه
Preparative scale enzymatic synthesis of D-sedoheptulose-7-phosphate from -hydroxypyruvate and D-ribose-5-phosphate
An enzymatic method for ready access to D-sedoheptulose-7-phosphate on a preparative scale was developed, based on the irreversible transketolase-catalyzed reaction: β-hydroxypyruvate + D-ribose-5phosphate → D-sedoheptulose-7-phosphate. D-Sedoheptulose-7-phosphate disodium salt was obtained in 81% overall yield determined using a standard curve obtained by LC/MS/MS.
متن کاملGenetic studies on ribose 5-phosphate isomerase mutants of Escherichia coli K-12.
A gene for the constitutive ribosephosphate isomerase (rpiA) is highly cotransducible with serA at 56.2 min on the genetic linkage map of Escherichia coli K-12. Suppression of ribosephosphate isomerase A-negative mutants can occur by a regulator gene mutation permitting constitutive synthesis of the normally inducible ribosephosphate isomerase B.
متن کاملInhibition of ribose-5-phosphate isomerase by 4-phosphoerythronate.
Hoping to exploit the special affinity of enzymes for unstable intermediates in substrate transformation, we have determined the effectiveness of possible analogs of ene-diolate intermediates as inhibitors of spinach ribose-5-phosphate isomerase. 4-Phosphoerythronic acid was found to be a very strong competitive inhibitor, with a Ki value almost 3 orders of magnitude lower than the Km value of ...
متن کاملThe control of pyruvate kinases of Escherichia coli: further studies of the enzyme activated by ribose-5-phosphate.
The pyruvate kinase activated by ribose-5-phosphate from Escherichia coli has been purified to homogeneity, taking advantage of the stabilization of the enzyme by its inhibitor phosphate and by thiol reagents. The native enzyme has a tetrameric quaternary structure which, while prone to dissociation under many conditions, remains intact in the presence of the above reagents. The enzyme was foun...
متن کاملD-ribose-5-phosphate isomerase B from Escherichia coli is also a functional D-allose-6-phosphate isomerase, while the Mycobacterium tuberculosis enzyme is not.
Interconversion of D-ribose-5-phosphate (R5P) and D-ribulose-5-phosphate is an important step in the pentose phosphate pathway. Two unrelated enzymes with R5P isomerase activity were first identified in Escherichia coli, RpiA and RpiB. In this organism, the essential 5-carbon sugars were thought to be processed by RpiA, while the primary role of RpiB was suggested to instead be interconversion ...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1955
ISSN: 0306-3283
DOI: 10.1042/bj0590322